Journal: Polymers

Article Title: Molecular Targets for Antibody-Based Anti-Biofilm Therapy in Infective Endocarditis

doi: 10.3390/polym14153198

Figure Lengend Snippet: Studies and clinical trials on potential anti-biofilm targets and antibodies. Adapted with permission from Raafat et al. [ 42 ]. 2019, Elsevier.

Article Snippet: LTA , Promote OPK , Murine/human chimeric mAb (Pagibaximab) , [ ] , Pagibaximab1 [Biosynexus; NCT00631800] , Phase II (finished) , Murine/human chimeric mAb , [ ] .

Techniques: Clinical Proteomics, Blocking Assay, Binding Assay, Bacteria, Activation Assay, Agglutination, Activity Assay, Recombinant, Shear

Journal: Molecular Cancer Research

Article Title: Role of Fibulin-5 in Metastatic Organ Colonization

doi: 10.1158/1541-7786.mcr-11-0093

Figure Lengend Snippet: Figure 4. Constitutive expression of Fbln5 stimulates motility and suppresses invasion of fibroblasts treated with CM from VMR mammary adenocarcinoma cells. A, closure of wounds of S100A4/ fibroblasts (MEFs) plotted as time postwounding. Left, the effect of Fbln5 on wound healing of MEF/Fbln5 cells compared with the vector controls in the absence (top) and presence (bottom) of VMR conditioned media (CM VMR). Right, the effect of Fbln5 knockdown on fibroblast wound closure. B, invasion of MEF/vector and MEF/Fbln5 cocultured with VMR cells in 3D Matrigel. C, invasion of MEF/vector, MEF/Fbln5 and MEF/shFbln5 in 3D Matrigel treated with VMR CM. D, the 3D Matrigel invasion of MEF/vector cells stimulated by VMR CM was blocked by the MMP2/MMP9 inhibitor SB-3CT (5 mmol/L). The lower panel in C and D shows the quantitative assessment of fibroblast invasion in the presence of VMR CM after 48 hours.

Article Snippet: Zymography and Western blot analysis Proteins were detected by using a standard Western blot procedure following SDS-PAGE Primary antibodies against MMP3 (Nordic Biosite), MMP9 (Biorbyt), and FBLN5 (BSYN 1923) were diluted according to the suppliers’ instructions.

Techniques: Expressing, Plasmid Preparation, Knockdown

Journal: Molecular Cancer Research

Article Title: Role of Fibulin-5 in Metastatic Organ Colonization

doi: 10.1158/1541-7786.mcr-11-0093

Figure Lengend Snippet: Figure 5. CM from VMR mammary adenocarcinoma cells induces expression of MMPs in fibroblasts under 3D Matrigel growth conditions. Zymography (A þ B) detecting active MMPs in 3D culture medium from MEF/vector and MEF/Fbln5 cells. A, b-casein zymography showing the presence of active MMP3 in CM of MEFs pretreated with VMR CM. B, gelatin zymography showing the presence of MMP2 and MMP9 activity in CM of MEF 3D cultures. Bottom, the increased intensity of MMP9-specific band in the MEF/vector cells stimulated by VMR CM in contrast to the MEF/Fbln5 treated cells. C, upregulation of Mmp3 transcription in 3D MEF culture stimulated with VMR CM (qRT-PCR analysis of RNA). Insert: Immunodetection of MMP3 in CM from MEF 3D cultures by Western blot analysis. D, activation of Mmp9 but not Mmp2 transcription in response to treatment with CM shown by qRT-PCR analysis. Insert, Western blot analyses of CM from corresponding samples detecting MMP9 and MMP2 protein.

Article Snippet: Zymography and Western blot analysis Proteins were detected by using a standard Western blot procedure following SDS-PAGE Primary antibodies against MMP3 (Nordic Biosite), MMP9 (Biorbyt), and FBLN5 (BSYN 1923) were diluted according to the suppliers’ instructions.

Techniques: Expressing, Zymography, Plasmid Preparation, Activity Assay, Quantitative RT-PCR, Immunodetection, Western Blot, Activation Assay

Journal: Molecular Cancer Research

Article Title: Role of Fibulin-5 in Metastatic Organ Colonization

doi: 10.1158/1541-7786.mcr-11-0093

Figure Lengend Snippet: Figure 6. Mmp9 expression is decreased in pulmonary lesions of VMR/Fbln5 tumors and in VMR/Fbln5 cells. A, immunohistochemical staining of lungs colonized by VMR/Fbln5 or VMR/vector tumor cells after intravenous injection into mice by using MMP9 antibodies. Representative sections from 3 animals are presented. B, decreased expression of Mmp9 and increased expression of Mmp3 in VMR/Fbln5 cells in comparison with VMR/vector control cells, determined by qRT-PCR analysis.

Article Snippet: Zymography and Western blot analysis Proteins were detected by using a standard Western blot procedure following SDS-PAGE Primary antibodies against MMP3 (Nordic Biosite), MMP9 (Biorbyt), and FBLN5 (BSYN 1923) were diluted according to the suppliers’ instructions.

Techniques: Expressing, Immunohistochemical staining, Staining, Plasmid Preparation, Injection, Comparison, Control, Quantitative RT-PCR